我校2004年1至8月发表在SCI源期刊上

科技论文目录（截止至2004年8月31日，共46篇)

Record 46 of 46

Report of a Chinese family with Marie-Unna hereditary hypotrichosis and exclusion of linkage at 8p21
Yan KL, He PP, Yang S, Li M, Yang Q, Ren YQ, Cui Y, Gao M, Xiao FL, Huang W, Zhang XJ
JOURNAL OF INVESTIGATIVE DERMATOLOGY

123 (2): 139 AUG 2004

Document type: Meeting Abstract   
Addresses:
AnhuiMedUniv, Inst Dermatol, Hefei, Peoples R China
AnhuiMedUniv, Hosp 1, Dept Dermatol, Hefei, Peoples R China
Chinese Natl Human Genome Ctr, Shanghai, Peoples R China
Key Lab Genome Res Anhui, Hefei, Peoples R China

Record 45of 46

Inhibitory effect of leflunomide on hepatic fibrosis induced by CCl4 in rats
Yao HW, Li J, Chen JQ, Xu SY
ACTA PHARMACOLOGICA SINICA

25 (7): 915-920 JUL 2004

Document type: Article   
Abstract:
AIM: To study the effect of leflunomide on CCl4-induced hepatic fibrosis in rats. METHODS: Hepatic fibrosis was induced by subcutaneous injection with 50 % CCl4, in Sprague-Dawley rats. The amount of CCl4 administered was 1 mg/kg. The alanine aminotransferase (ALT), aspartate aminotransferase (AST), nitric oxide (NO) levels in plasma and hydroxyproline (Hyp) contents in liver tissue were assayed by spectrophotometry. The hyaluronic acid (HA) and procollagen III (PC 111) were assessed by radioimmunoassay. The transforming growth factor-beta1 (TGF-beta1) in serum was determined by ELISA. The nuclear factor-kappa B (NF-kappaB) in liver tissue was examined by immunohistochemistry. Liver samples collected after 12 weeks of CCl4 treatment were stained with hematoxylin and eosin. RESULTS: Leflunomide (1, 3, and 9 mg/kg) significantly decreased indices of liver and spleen, the serum transaminase (AST, ALT) activities, HA and PC III levels, and Hyp contents in liver tissue in rats of hepatic fibrosis. Histopathological examination showed leflunomide had inhibitory effect on fibrogenesis and formation of pseudolobulus. Furthermore, leflunomide significantly inhibited NF-kappaB expression in liver tissue, and reduced elevated serum TGF-beta1 and NO levels in rats of hepatic fibrosis. CONCLUSION: Leflunomide showed inhibitory action on hepatic fibrosis induced by CCl4 in rats.

Author Keywords:
leflunomide, hepatic fibrosis, nuclear factor-kappa B, transforming growth factor-beta, nitric oxide

KeyWords Plus:
NF-KAPPA-B, NITRIC-OXIDE SYNTHASE, LIVER FIBROSIS, STELLATE CELLS, ACTIVATION, APOPTOSIS, PHARMACOKINETICS, RESPONSES, INJURY, MODELS

Addresses:
Li J, Zhejiang Univ, Sch Med, Zhejiang Resp Drugs Res Lab State Drugs Adm China, Hangzhou 310031, Peoples R China
Zhejiang Univ, Sch Med, Zhejiang Resp Drugs Res Lab State Drugs Adm China, Hangzhou 310031, Peoples R China
AnhuiMedUniv, Inst Clin Pharmacol, Hefei 230032, Peoples R China

Record 44of 46

Identification of the cylindromatosis tumor-suppressor gene responsible for multiple familial trichoepithelioma
Zhang XJ, Liang YH, He PP, Yang S, Wang HY, Chen JJ, Yuan WT, Xu SJ, Cui Y, Huang W
JOURNAL OF INVESTIGATIVE DERMATOLOGY

122 (3): 658-664 MAR 2004

Document type: Article   
Abstract:
Multiple familial trichoepithelioma (MFT) is an autosomal dominant skin disease characterized by the presence of many small benign tumors with pilar differentiation predominantly on the face. The first locus has been previously mapped to chromosome 9p21, but no gene for MFT has been identified to date. To identify the disease gene in a large Chinese family, we initially performed linkage analysis with microsatellite markers from 9p21, but failed to confirm the linkage to this region. Previous publications showed MFT and familial cylindromatosis (FC) can occur within one family and in a single person. Therefore, we speculated that the cylindromatosis gene (CYLDI gene) responsible for FC may be related to the pathogenesis of MFT. In view of that, we genotyped all available individuals using 11 microsatellite markers spanning the CYLDI gene region at 16q12-q13. We identified the linkage of MFT to this region. Mutation analysis in the CYLDI gene detected a frameshift mutation, designated as c.2355-2358delCAGA. The study firstly identified the cylindromatosis gene responsible for MFT and showed that different mutations of the CYLDI gene can give rise to distinct clinical and histological expression such as FC and MFT.

Author Keywords:
CYLD1 gene, heterogeneity, linkage analysis, multiple familial trichoepithelioma, mutation

KeyWords Plus:
BASAL-CELL CARCINOMA, CHROMOSOME 16Q12-Q13, LOCUS, CYLD, MUTATION, SCORES

Addresses:
Zhang XJ, AnhuiMedUniv, Inst Dermatol, Hefei, Peoples R China
AnhuiMedUniv, Inst Dermatol, Hefei, Peoples R China
Key Lab Genome Res Anhui, Hefei, Peoples R China
Chinese Natl Human Genome Ctr Shanghai, Shanghai, Peoples R China

Record 43of 46

Haplotype associations of the MHC with psoriasis vulgaris in Chinese Hans
Yang S, Ge H, Zhang A, Wei S, Gao M, Wang H, Chen J, Li M, Liang Y, He P, Yang J, Zhang X
JOURNAL OF INVESTIGATIVE DERMATOLOGY

122 (3): 037 MAR 2004

Document type: Meeting Abstract   
Addresses:
AnhuiMedUniv, Affiliated Hosp 1, Inst Dermatol, Hefei, Anhui, Peoples R China
AnhuiMedUniv, Affiliated Hosp 1, Dept Dermatol, Hefei, Anhui, Peoples R China

Record 42 of 46

The genetic epidemiology of alopecia areata in Chinese
Yang J, Yang S, Liu J, Wang H, Yang Q, Gao M, Liang Y, Lin G, Lin D, Hu X, Fan L, Zhang X
JOURNAL OF INVESTIGATIVE DERMATOLOGY

122 (3): 365 MAR 2004

Document type: Meeting Abstract   
Addresses:
AnhuiMedUniv, Inst Dermatol, Hefei, Anhui, Peoples R China
AnhuiMedUniv, Dept Dermatol, Affiliated Hosp 1, Hefei, Anhui, Peoples R China
Key Lab Genome Res Anhui, Hefei, Anhui, Peoples R China

Record 41of 46

Refinement of dyschromatosis symmetrica hereditaria locus to a 9.4-cM interval at 1q21-q22 and a literature review of Chinese patients with this disorder
He P, He C, Cui Y, Yang S, Xu H, Li M, Yuan W, Gao M, Liang Y, Li C, Xu S, Chen J, Chen H, Huang W, Zhang X
JOURNAL OF INVESTIGATIVE DERMATOLOGY

122 (3): 491 MAR 2004

Document type: Meeting Abstract   
Addresses:
AnhuiMedUniv, Inst Dermatol, Hefei, Anhui, Peoples R China
AnhuiMedUniv, Affiliated Hosp 1, Dept Dermatol, Hefei, Anhui, Peoples R China
Chinese Natl Human Genome Ctr Shanghai, Shanghai, Peoples R China
China Med Univ, Hosp 1, Dept Dermatol, Shenyang, Liaoning, Peoples R China
Key Lab Genome Res Anhui, Hefei, Anhui, Peoples R China

Record 40of 46

Identification of a novel missense mutation of ATP2A2 in a Chinese family with Darier's
Sun L, Yang S, Liu H, Wang J, He P, Li M, Gao M, Liu J, Yang J, Wang Z, Zhu Y, Lin D, Zhang X
JOURNAL OF INVESTIGATIVE DERMATOLOGY

122 (3): 488 MAR 2004

Document type: Meeting Abstract   

Addresses:
AnhuiMedUniv, Inst Dermatol, Hefei, Anhui, Peoples R China
AnhuiMedUniv, Affiliated Hosp 1, Dept Dermatol, Hefei, Anhui, Peoples R China
Key Lab Genome Res Anhui, Hefei, Anhui, Peoples R China
China Med Univ, Hosp 1, Dept Dermatol, Shenyang, Liaoning, Peoples R China

Record 39of 46

Identification of seven novel mutations in the ADAR gene among Chinese patients with dyschromatosis symmetrica hereditaria
Zhang X, He P, Li M, He C, Yan K, Cui Y, Yang S, Zhang K, Gao M, Chen J, Jin L, Chen H, Xu S, Huang W
JOURNAL OF INVESTIGATIVE DERMATOLOGY

122 (3): 490 MAR 2004

Document type: Meeting Abstract   
Addresses:
AnhuiMedUniv, Inst Dermatol, Hefei, Anhui, Peoples R China
AnhuiMedUniv, Affilaited Hosp 1, Dept Dermatol, Hefei, Anhui, Peoples R China
Key Lab Genome Res Anhui, Hefei, Anhui, Peoples R China
Chinese Natl Human Genome Ctr Shanghai, Shanghai, Peoples R China
China Med Univ, Hosp 1, Dept Dermatol, Shenyang, Liaoning, Peoples R China

Record 38of46

A refined locus for marie unna hereditary hypotrichosis1.1-cM: interval at 8p21.3
Liang Y, He P, Zhang X, Yang Q, Li M, Yang S, Yan K, Cui Y, Shen Y, Wang H, Sun L, Du W, Shen Y, Xu S, Huang W
JOURNAL OF INVESTIGATIVE DERMATOLOGY

122 (3): 492 MAR 2004

Document type: Meeting Abstract   
Addresses:
Chinese Natl Human Genome Ctr, Shanghai, Peoples R China
AnhuiMedUniv, Affiliated Hosp 1, Hefei, Peoples R China
Key Lab Genome Res Anhui, Hefei, Anhui, Peoples R China

Record 37of 46

Association of HLA-A, B, C alleles with vitiligo in Chinese population
Liu H, Zhang X, Sun L, Wang J, Yang S, Liu J, Liang Y, Gao M, He P, Zhang A, Cui Y
JOURNAL OF INVESTIGATIVE DERMATOLOGY

122 (3): 893 MAR 2004

Document type: Meeting Abstract   
Addresses:
AnhuiMedUniv, Inst Dermatol, Hefei, Anhui, Peoples R China
AnhuiMedUniv, Affiliated Hosp 1, Dept Dermatol, Hefei, Anhui, Peoples R China
Key Lab Genome Res Anhui, Hefei, Anhui, Peoples R China

Record 36of 46

Human leukocyte antigen DQA1 and DQB1 alleles are associated with genetic susceptibility to vitiligo in Chinese
Wang J, Yang S, Gao M, Liu H, Sun L, He P, Liu J, Zhang A, Cui Y, Liang Y, Wang Z, Zhang X
JOURNAL OF INVESTIGATIVE DERMATOLOGY

122 (3): 892 MAR 2004

Document type: Meeting Abstract   
Addresses:
AnhuiMedUniv, Inst Dermatol, Hefei, Anhui, Peoples R China
AnhuiMedUniv, Affiliated Hosp 1, Dept Dermatol, Hefei, Anhui, Peoples R China
Key Lab Genome Res Anhui, Hefei, Anhui, Peoples R China
Inst Prevent & Cure Skin Dis Anhui, Hefei, Anhui, Peoples R China

Record 35of 46

The genetic epidemiology and the mode of inheritance of vitiligo
Liu J, Li M, Gui J, Xiong Q, Yang S, Wang H, Gao M, Yang J, Yang Q, Zhang X
JOURNAL OF INVESTIGATIVE DERMATOLOGY

122 (3): 894 MAR 2004

Document type: Meeting Abstract   
Addresses:
AnhuiMedUniv, Inst Dermatol, Hefei, Anhui, Peoples R China
AnhuiMedUniv, Affiliated Hosp 1, Dept Dermatol, Hefei, Anhui, Peoples R China
Key Lab Genome Res Anhui, Hefei, Anhui, Peoples R China
Xiangfan Railway Hosp, Inst Vitiligo, Xiangfan, Hubei, Peoples R China

Record 34of 46

The study of the participation of basement membrane zone antibodies in the formation of the lupus band in systemic lupus erythematosus
Yang S, Gao Y, Song YX, Liu JB, Yang CJ, Liu JL, Wang ZX, Zhang XJ
INTERNATIONAL JOURNAL OF DERMATOLOGY

43 (6): 420-427 JUN 2004

Document type: Article   
Abstract:
Background The lupus band test (LBT) is an important auxiliary method in the diagnosis of systemic lupus erythematosus (SLE), but the mechanism of its formation is still unknown. There are many kinds of autoantibodies, such as basement membrane zone autoantibodies (BMZ-Abs), in patients with SLE.

Aim To detect whether skin BMZ-Abs participated in the formation of the lupus band in SLE.

Methods Immunoglobulin G (IgG)-type BMZ-Abs in 15 SLE patients were detected by means of immunofluorescence (IF), immunoblotting (113), and immunoelectron microscopy (IEM).

Results Direct immunofluorescence (DIF) on salt-split skin showed epidermal fluorescence in 12 of the 15 SLE patients. Two of the 12 patients also showed dermal fluorescence. Indirect immunofluorescence (IIF) on salt-split skin revealed that, in 12 of the 15 (80%) sera, antibodies were bound to the epidermal roof of the salt-split skin. IB showed that, in 14 SLE sera, autoantibodies reacted to 230, 200, 180, 130, and 97 kDa epidermal extracts and 75 kDa dermal extracts. Direct (DIEM) and indirect (IIEM) immunoelectron microscopy showed that gold particles were directed to every region of the BMZ, including hemidesmosomes, lamina lucida, lamina densa, and sublamina densa.

Conclusions BMZ-Abs in SLE sera may participate in the formation of the lupus band.

KeyWords Plus:
ANTI-DNA COMPLEXES, REVISED CRITERIA, SKIN, IMMUNOFLUORESCENCE, AUTOANTIBODIES, CLASSIFICATION, COLLAGEN, LESIONS

Addresses:
Zhang XJ, AnhuiMedUniv, Inst Dermatol, Hefei 230032, Anhui, Peoples R China
AnhuiMedUniv, Inst Dermatol, Hefei 230032, Anhui, Peoples R China

Record 33of 46

A 771726, the active metabolite of leflunomide, inhibits TNF-alpha and IL-1 from Kupffer cells
Yao HW, Li J, Chen JQ, Xu SY
INFLAMMATION

28 (2): 97-103 APR 2004

Document type: Article   
Abstract:
This study was conducted to investigate the importance of direct contact between Kupffer cells (KCs) and hepatocytes (HCs) during the hepatic inflammatory responses, and the effect of leflunomide's active metabolite, A771726, on cytokines in KCs and HCs (DC cocultures) and KC cultures using an in vitro approach. Lipopolysaccharide (LPS)-induced inflammatory response in monocultures of rats HCs and KCs were compared with DC cocultures. Tumor necrosis factor-alpha (TNF-alpha) and interleukin-1 (IL-1) concentrations of different culture supernatants were measured with ELISA. TNF-alpha and IL-1 mRNA in KCs of inflammatory liver injury was analyzed with reverse transcription polymerase chain reaction (RT-PCR). Our data showed that DC cocultures exhibited the highest production of TNF-alpha and IL-1 compared with other cultures, and these cytokines were mainly produced by KCs, in particular activated KCs. Time course studies revealed an increased production of TNF-alpha preceding the IL-1 production, suggesting that increased TNF-alpha levels could be involved in the increased IL-1 production. Leflunomide's active metabolite, A771726, has significantly inhibitory effect on TNF-alpha and IL-1 at protein and transcription levels, and reduced production of IL-1 by A771726 was associated with inhibitory action of A771726 on TNF-alpha. These results provided evidence that leflunomide significantly inhibited TNF-alpha and IL-1 from KCs.

Author Keywords:
leflunomide, A771726, lipopolysaccharide, cytokines, Kupffer cells, hepatocytes

KeyWords Plus:
INDUCED LIVER-INJURY, KAPPA-B, PHARMACOKINETICS, MICE

Addresses:
Li J, AnhuiMedUniv, Sch Pharm, Hefei 230032, Peoples R China
AnhuiMedUniv, Sch Pharm, Hefei 230032, Peoples R China
Zhejiang Univ, Sch Med, Zhejiang Resp Drugs Res Lab State Drugs Adm China, Hangzhou, Peoples R China

Record 32of 46

D919G polymorphism of methionine synthase gene is associated with blood pressure response to benazepril in Chinese hypertensive patients
Zhang Y, Zhang MM, Niu TH, Xu X, Zhu GY, Huo Y, Chen CZ, Wang XB, Xing HX, Peng SJ, Huang AQ, Hong XM, Xu XP
JOURNAL OF HUMAN GENETICS

49 (6): 296-301 JUN 2004

Document type: Article   
Abstract:
Individual variation in drug response is considered to have multiple origins arising from interactions among susceptible genes and environmental factors. A total of 726 hypertensive patients who took benazepril 10 mg once a day for 15 days and their families from Huoqiu county of Anhui Province, China, were used to study the association between D919G polymorphism of methionine synthase (MTR) gene and the antihypertensive effect of this angiotensin-converting enzyme inhibitor. Compared to the 919D allele, both population-based (P=0.010) and family-based association tests (additive model P=0.018, dominant model P=0.025) demonstrated that the 919G allele was associated with a significantly less diastolic blood pressure reduction. No significant association was found between the extent of systolic blood pressure reduction and benazepril therapy. Our finding suggests that the D919G polymorphism of the MTR gene may be a useful genetic marker to predict the antihypertensive effect of short-term benazepril therapy in hypertensive patients of Anhui Province, China.

Author Keywords:
methionine synthase, polymorphism, hypertension, blood pressure, benazepril

KeyWords Plus:
PLASMA HOMOCYSTEINE, METHYLENETETRAHYDROFOLATE REDUCTASE, ENDOTHELIAL DYSFUNCTION, MYOCARDIAL-INFARCTION, COMMON MUTATION, RISK FACTOR, STIFFNESS, HYPERHOMOCYSTEINEMIA, HOMOCYST(E)INE, POPULATION

Addresses:
Xu X, Harvard Univ, Sch Publ Hlth, Program Populat Genet, 665 Huntington Ave,FXB-101, Boston, MA 02115 USA
Harvard Univ, Sch Publ Hlth, Program Populat Genet, Boston, MA 02115 USA
Peking Univ, Hosp 1, Dept Cardiol, Beijing 100034, Peoples R China
Univ Sci & Technol China, Sch Life Sci, Hefei 230026, Peoples R China
Harvard Univ, Sch Med, Brigham & Womens Hosp, Dept Med,Div Prevent Med, Boston, MA 02215 USA
Childrens Mem Hosp, Mary Ann & J Milburn Smith Child Hlth Program, Chicago, IL 60614 USA
Childrens Mem Inst Educ & Res, Chicago, IL 60614 USA
AnhuiMedUniv, Inst Biomed, Hefei, Peoples R China

Record 31of 46

Elasticity of small artery in patient with spontaneous rupture of hepatocellular carcinoma
Zhu LX, Meng XL, Fan ST
HEPATOLOGY RESEARCH

29 (1): 13-17 MAY 2004

Document type: Article   
Abstract:
Objective: Spontaneous rupture of hepatocellular carcinoma (HCC) is common in Asia and Africa with unclear mechanism. In our previous study, we found that the vascular injury was related to the HCC rupture. In this study, the structure of elastin around the small artery was deeply investigated to confirm our previous study. Methods: Immunohistochemical technique and transmission electron microscopy were used to study 23 specimens from ruptured HCC and 30 cases with nonruptured HCC. Results: The layer of elastin around the vascular wall was significant thicker in patients with ruptured HCC than that in nonruptured HCC. The proliferation of elastin, abnormal distribution of neutrophil elastase and degradation of collagen fibril were predominantly present in the specimens from ruptured HCC. The phenomenon that the infiltrated neutrophils from bloodstream into the vascular wall, which caused the vascular injury, can be found in specimens from ruptured HCC. The vascular injury mainly occurred in small artery. Since the damaged vessels could become stiff and weak, which would be more prone to splitting and result in hemorrhage in patients with ruptured HCC, we postulated that the vascular injury, especially the inelastic small artery, may relate the ruptured HCC. Conclusion: The vascular injury in small artery might relate to ruptured HCC. (C) 2004 Elsevier B.V. All rights reserved.

Author Keywords:
hepatocellular carcinoma, rupture, elastin, elastase

KeyWords Plus:
INJURY

Addresses:
Zhu LX, AnhuiMedUniv, Affiliated Hosp, Dept Surg, Hefei 230022, Anhui, Peoples R China
AnhuiMedUniv, Affiliated Hosp, Dept Surg, Hefei 230022, Anhui, Peoples R China
Univ Hong Kong, Dept Surg, Hong Kong, Hong Kong, Peoples R China

Record 30of 46

Kupffer cells and reactive oxygen species partially mediate lipopolysaccharide-induced downregulation of nuclear receptor pregnane X receptor and its target gene CYP3A in mouse liver
Xu DX, Wei W, Sun MF, Wu CY, Wang JP, Wei LZ, Zhou CF
FREE RADICAL BIOLOGY AND MEDICINE

37 (1): 10-22 JUL 1 2004

Document type: Article   
Abstract:
Pregnane X receptor (PXR) is a member of the nuclear receptor superfamily that regulates target gene transcription in a ligand-dependent manner. The in vivo effects of lipopolysaccharide (LPS) on expression of PXR and its target gene cytochrome P450 3A (CYP3A) in mouse liver were investigated in this study. Mice were injected intraperitoneally with different doses of LPS (0.1-5.0 mg/kg). PXR and CYP3A11 mRNA levels were measured using reverse transcription polymerase chain reaction. Results indicate that LPS significantly inhibits the expression of PXR mRNA in a dose-dependent manner, followed by suppression of CYP3A11 mRNA in mouse liver. LPS also represses the upregulation of CYP3A11 mRNA levels and erythromycin N-demethylase (ERND) catalytic activity in mice pretreated with PXR ligands dexamethasone, rifampicin, mifiepristone, and phenobarbital. LPS-induced downregulation of PXR and CYP3A 11 mRNA in liver was significantly attenuated in mice pretreated with gadolinium chloride, a selective Kupffer cell toxicant. Pretreatment with a single dose of gadolinium chloride (10 mg/kg) also significantly attenuated LPS-induced downregulation of dexamethasone-, rifampicim-, mifepristone-, and phenobarbital-inducible, CYP3A I I mRNA expression and ERND activity in mouse liver. Furthermore, LPS-induced downregulation of PXR and CYP3A11 mRNA was significantly attenuated in mice pretreated with allopurinol, an inhibitor of xanthine oxidase, and diphenyleneiodonium chloride, an inhibitor of NADPH oxidase. Allopurinol and diphenyleneiodonium chloride pretreatment also attenuated the repressive effects of LPS on dexamethasone-, rifampicin-, mifepristone-, and phenobarbital-inducible CYP3A H mRNA expression and ERND catalytic activity in mouse liver. However, aminoguanidine, a selective inhibitor of inducible nitric oxide synthase, has no effect on LPS-induced downregulation of PXR and CYP3A11 mRNA. Finally, LPS-induced downregulation of PXR and CYP3A I I mRNA was prevented in mice pretreated with either N-acetylcysteme or ascorbic acid. These antioxidants also prevented the repressive effects of LPS on dexamethasone-, rifampicin-, mifepristone-, and phenobarbital-inducible CYP3A11 mRNA expression and ERND catalytic activity in mouse liver. These results indicate that Kupffer cells contribute to LPS-induced downregulation of PXR and CYP3A in mouse liver. Reactive oxygen species, produced possibly by NADPH oxidase and perhaps by xanthine oxidase, are involved in LPS-induced downregulation of nuclear receptor PXR and its target gene CYP3A in mouse liver. (C) 2004 Elsevier Inc. All rights reserved.

Author Keywords:
lipopolysaccharide, pregnarie X receptor, cytochrome P450 3A, Kupffer cells, reactive oxygen species, antioxidants, free radicals

KeyWords Plus:
NITRIC-OXIDE SYNTHASE, NF-KAPPA-B, NECROSIS-FACTOR-ALPHA, IN-VIVO, RAT-LIVER, SIGNALING PATHWAY, DRUG-INTERACTIONS, HUMAN HEPATOCYTES, OXIDATIVE STRESS, P450 2C11

Addresses:
Xu DX, AnhuiMedUniv, Inst Clin Pharmacol, Hefei 230032, Peoples R China
AnhuiMedUniv, Inst Clin Pharmacol, Hefei 230032, Peoples R China
AnhuiMedUniv, Dept Toxicol, Hefei 230032, Peoples R China

Record 29of 46

A novel missense mutation of the ATP2A2 gene in a Chinese family with Darier's disease
Yang S, Sun LD, Liu HS, Wang JY, He PP, Li M, Gao M, Liu JB, Yang J, Wang ZX, Zhu YY, Lin D, Zhang XJ
ARCHIVES OF DERMATOLOGICAL RESEARCH

296 (1): 21-24 JUN 2004

Document type: Article   
Abstract:
Darier's disease (DD) is an autosomal dominant skin disorder that is characterized by multiple keratotic papules, focal loss of adhesion and abnormal keratinization. Mutations in the ATP2A2 gene encoding sarco/endoplasmic reticulum calcium pumping ATPase type 2 have been identified as the molecular basis of DD. We report here a three-generation family with DD, and examined ATP2A2 gene mutations in this family by direct sequencing. A novel missense mutation A-->G was identified in exon 12, nucleotide 1704, which leads to the substitution of lysine by arginine at codon 514 (K514R). This study contributes to the database on ATP2A2 in DD, and further illustrates the extensive diversity of mutational events that lead to the different phenotypes of DD.

Author Keywords:
Darier's disease, mutation analysis, ATP2A2

KeyWords Plus:
INTRACELLULAR CALCIUM STORES, RETICULUM, PHENOTYPE

Addresses:
Zhang XJ, AnhuiMedUniv, Inst Dermatol, 69 Meishan Rd, Hefei 2300032, Anhui, Peoples R China
AnhuiMedUniv, Inst Dermatol, Hefei 2300032, Anhui, Peoples R China
AnhuiMedUniv, Hosp 1, Dept Dermatol, Hefei, Peoples R China
Key Lab Genome Res Anhui, Hefei, Peoples R China

Record 28of 46

A common haplotype of the nicotine acetylcholine receptor alpha 4 subunit gene is associated with vulnerability to nicotine addiction in men
Feng Y, Niu TH, Xing HX, Xu X, Chen CZ, Peng SJ, Wang LH, Laird N, Xu XP
AMERICAN JOURNAL OF HUMAN GENETICS

75 (1): 112-121 JUL 2004

Document type: Article   
Abstract:
Nicotine is the major addictive substance in cigarettes, and genes involved in sensing nicotine are logical candidates for vulnerability to nicotine addiction. We studied six single-nucleotide polymorphisms (SNPs) in the CHRNA4 gene and four SNPs in the CHRNB2 gene with respect to nicotine dependence in a collection of 901 subjects (815 siblings and 86 parents) from 222 nuclear families with multiple nicotine-addicted siblings. The subjects were assessed for addiction by both the Fagerstrom Test for Nicotine Dependence (FTND) and the Revised Tolerance Questionnaire (RTQ). Because only 5.8% of female offspring were smokers, only male subjects were included in the final analyses ( 621 men from 206 families). Univariate (single-marker) family-based association tests (FBATs) demonstrated that variant alleles at two SNPs, rs1044396 and rs1044397, in exon 5 of the CHRNA4 gene were significantly associated with a protective effect against nicotine addiction as either a dichotomized trait or a quantitative phenotype (i.e., age-adjusted FTND and RTQ scores), which was consistent with the results of the global haplotype FBAT. Furthermore, the haplotype-specific FBAT showed a common (22.5%) CHRNA4 haplotype, GCTATA, which was significantly associated with both a protective effect against nicotine addiction as a dichotomized trait (Z = -3.04, P < .005) and significant decreases of age-adjusted FTND (Z = -3.31, P < .005) or RTQ scores (Z = -2.73, P = .006). Our findings provide strong evidence suggesting a common CHRNA4 haplotype might be protective against vulnerability to nicotine addiction in men.

KeyWords Plus:
FRONTAL-LOBE EPILEPSY, FAGERSTROM TOLERANCE QUESTIONNAIRE, SINGLE NUCLEOTIDE POLYMORPHISMS, FAMILY-BASED TESTS, SMOKING INITIATION, DEPENDENCE, CHRNA4, DISORDER, MUTATION, ANXIETY

Addresses:
Niu TH, Harvard Univ, Sch Med, Brigham & Womens Hosp, Dept Med,Div Prevent Med, 900 Commonwealth Ave, Boston, MA 02215 USA
Harvard Univ, Sch Med, Brigham & Womens Hosp, Dept Med,Div Prevent Med, Boston, MA 02215 USA
Harvard Univ, Sch Publ Hlth, Program Populat Genet, Boston, MA 02115 USA
Harvard Univ, Sch Publ Hlth, Dept Biostat, Boston, MA 02115 USA
Peking Univ, Hlth Sci Ctr, Ctr Ecogenet & Reprod Hlth, Beijing 100871, Peoples R China
AnhuiMedUniv, Anhui Inst Biomed, Hefei, Anhui, Peoples R China
Univ Sci & Technol China, Sch Life Sci, Hefei 230026, Anhui, Peoples R China

Record 27of 46

A surgical approach to middle ear can preserve rat cochlear function
Qiu JX, Duan ML
CHINESE MEDICAL JOURNAL

117 (5): 767-769 MAY 2004

Document type: Article   
Author Keywords:
surgical anatomy, scala tympani, middle ear, rat

KeyWords Plus:
OTITIS-MEDIA, BLOOD-FLOW, ANATOMY, MEMBRANE

Addresses:
Qiu JX, AnhuiMedUniv, Affiliated Hosp 1, Dept Otolaryngol, Hefei 230022, Peoples R China
AnhuiMedUniv, Affiliated Hosp 1, Dept Otolaryngol, Hefei 230022, Peoples R China

Record 26of 46

A community-based study of depression in older people in Hefei, China - the GMS-AGECAT prevalence, case validation and socio-economic correlates
Chen RL, Hu Z, Qin X, Xu XC, Copeland JRM
INTERNATIONAL JOURNAL OF GERIATRIC PSYCHIATRY

19 (5): 407-413 MAY 2004

Document type: Article   
Abstract:
Objective: Previously published studies of prevalence of depression in older people in China showed much variation in the results, while the pooled prevalence is low. In this study we used a standardised method, the Geriatric Mental State (GMS) to investigate the prevalence of depression in older people in China, validate the GMS-AGECAT depression cases and examine the relationship between depression and socio-economic deprivation.

Methods: 1736 subjects aged greater than or equal to 65 were recruited from Hefei city, China. They were interviewed at home by a survey team from Anhui Medical Universtiy using the GMS and other interviews. Their mental disorders were diagnosed by the Automated Geriatric Examination for Computer Assisted Taxonomy (AGECAT). Chinese psychiatrists re-examined depression cases and their controls.

Results: 39 depressed cases were diagnosed by the GMS-AGECAT. Age-standardised prevalence was 2.2% (95% CI 1.5-2.9), which was about five-times lower than that of older people in Liverpool, UK. Agreement on depression diagnoses between the GMS-AGECAT and local Chinese psychiatrists was 83.6%, with a Kappa of 0.67 (p < 0.001). Depression was significantly related to socio-economic deprivation.

Conclusions: This community-based study suggested a low prevalence of depression in older people in urban China. Approved training in the use of the GMS-AGECAT in mainland China should make it possible to carry out a large scale epidemiological study on depression in the Chinese elderly population to investigate its geographic variation and risk factors. The dose-response relation between socio-economic deprivation and depression indicates that strategies for tackling inequality in depression in elderly are urgently needed in China. Copyright (C) 2004 John Wiley Sons, Ltd.

Author Keywords:
Chinese, community, depression, elderly, GMS-AGECAT, socio-economic deprivation, validation

KeyWords Plus:
STRUCTURED CLINICAL INTERVIEW, MENTAL STATE SCHEDULE, MRC-ALPHA, DIAGNOSIS, DEMENTIA, METAANALYSIS, RELIABILITY, LIFE

Addresses:
Chen RL, Univ Coll London, Int Ctr Hlth & Soc, Dept Epidemiol & Publ Hlth, 1-19 Torrington Pl, London WC1E 6BT, England
Univ Coll London, Int Ctr Hlth & Soc, Dept Epidemiol & Publ Hlth, London WC1E 6BT, England
AnhuiMedUniv, Sch Hlth Adm, Hefei, Peoples R China
Univ Liverpool, Dept Psychiat, Liverpool L69 3BX, Merseyside, England

Record 25of 46

Eating more vegetables might explain reduced asthma symptoms
Chen RL, Hu Z, Seaton A
BRITISH MEDICAL JOURNAL

328 (7452): 1380-1380 JUN 5 2004

Document type: Letter   
KeyWords Plus:
TRENDS

Addresses:
Chen RL, Univ Coll London, Dept Epidemiol & Publ Hlth, Mortimer St, London WC1E 6BT, England
Univ Coll London, Dept Epidemiol & Publ Hlth, London WC1E 6BT, England
AnhuiMedUniv, Sch Hlth Adm, Hefei 230032, Anhui, Peoples R China

Record 24of 46

Aberration of CCR7(+) CD8(+) memory T cells from patients with systemic lupus erythematosus: an inducer of T helper type 2 bias of CD4(+) T cells
Yang S, Hu CS, Huang BJ, Zhang LJ, Li Q, Jiang S, Zhang QP, Liu JY, Xuejun Z, Tan JQ
IMMUNOLOGY

112 (2): 274-289 JUN 2004

Document type: Article   
Abstract:
Chemokine receptors are important in the entry of leucocytes into the inflammatory sites of systemic lupus erythematosus (SLE). CCR7(+) and CCR7(-) memory T cells exert different functions in homing, cytokine production and cytotoxicity. To determine whether differential expression and functions of the CCR7 occur in SLE patients, we examined CCR3, CCR4, CCR5, CCR7 and CCR9 on CD4(+) and CD8(+) T cells from normal and SLE subjects. Flow cytometry, real-time quantitative reverse transcription polymerase chain reactions and Northern blotting were used to detect the expression of chemokine receptors and cytokines; a chemotaxis assay was used to detect their functions. CD4(+) T-cell stimulation with syngeneic CCR7(+) CD8(+) CD45RO(+) T cells and dendritic cells (including transwell chambers) was used to induce cytokine expression. We demonstrated that CCR7 was selectively, frequently and functionally expressed on CD8(+) (94.8%) but not on CD4(+) (16.1%) T cells from patients with active SLE, whereas this phenomenon was not seen in normal subjects and in those whose SLE was inactive. CCR7(+) CD8(+) CD45RO(+) memory T cells from patients with active SLE, themselves T helper type 2 (Th2) biased, were inducers of Th2 bias in CD4(+) T cells in a cell-cell contact manner in vitro, meanwhile, the cells from both normal subjects and those whose SLE was inactive drove CD4(+) T cells into a regulatory T-cell-derived cytokine pattern. Our findings might provide new clues to understanding the functions of CCR7(+) CD8(+) CD45RO(+)'central' memory T cells in autoimmue diseases (such as SLE). We suggest that in the case of active SLE, CCR7(+) central memory T cells were able to enter peripheral blood and inflammatory sites from secondary lymphoid organs, were continuously expressing CCR7, and interacted with dendritic cells and functioned as CCR7(-)'effector' memory T cells, which were described in normal humans.

Author Keywords:
chemokine receptors : CCR7, systemic lupus erythematosus (SLE), T cells : memory cells, Th2 cells

KeyWords Plus:
SECONDARY LYMPHOID ORGANS, HUMAN DENDRITIC CELLS, IFN-GAMMA, CHEMOKINE RECEPTORS, INTERFERON-GAMMA, MURINE LUPUS, REGULATORY PROPERTIES, TRANSGENIC MICE, IMMUNE-RESPONSE, TNF-ALPHA

Addresses:
Tan JQ, Wuhan Univ, Coll Med, Dept Immunol, Dong Hu Rd 115, Wuhan 430071, Peoples R China
Wuhan Univ, Coll Med, Dept Immunol, Wuhan 430071, Peoples R China
AnhuiMedUniv, Affiliated Univ Hosp, Inst Dermatol, Hefei, Peoples R China
AnhuiMedUniv, Coll Basic Med Sci, Dept Immunol, Hefei, Peoples R China

Record 23of 46

Refinement of a locus for Marie Unna hereditary hypotrichosis to a 1.1-cM interval at 8p21.3
He PP, Zhang XJ, Yang Q, Li M, Liang YH, Yang S, Yan KL, Cui Y, Shen YY, Wang HY, Sun LD, Du WH, Shen YJ, Xu SJ, Huang W
BRITISH JOURNAL OF DERMATOLOGY

150 (5): 837-842 MAY 2004

Document type: Article   
Abstract:
Background Marie Unna hereditary hypotrichosis (MUHH) is a rare autosomal congenital alopecia with progressive hair loss starting in early childhood and accelerating at puberty. A locus for MUHH has been mapped on chromosome 8p21 but no genes for MUHH have been identified to date.

Objectives To refine the MUHH locus to a narrow chromosome region to facilitate cloning of the gene.

Methods We performed genotyping and linkage analysis in a multigeneration Chinese family with MUHH, using 18 high-density microsatellite markers spanning the previously mapped interval at 8p21.

Results Significant evidence for linkage was observed in this region, with a maximum two-point LOD score of 3.01 (theta = 0). Haplotype analysis localized the MUHH locus within the region defined by D8S282 and D8S1839. This region overlaps by 1.1-cM with the previously reported MUHH region and represents a physical distance of about 380 kb.

Conclusions This study provides a refined map location (1.1 cM) for isolation of the gene causing MUHH. These data also indicate the existence of a common MUHH locus at 8p21.3 between affected caucasian and Chinese families.

Author Keywords:
haplotype, linkage analysis, Marie Unna hereditary hypotrichosis, refined mapping

KeyWords Plus:
HUMAN HAIRLESS GENE, MARIE-UNNA, CONGENITAL ATRICHIA, CHROMOSOME 8P21, PAPULAR LESIONS, MUTATION, EXCLUSION, ALOPECIA, PEDIGREE, SCORES

Addresses:
Zhang XJ, AnhuiMedUniv, Inst Dermatol, 69 Meishan Rd, Hefei 230032, Anhui, Peoples R China
AnhuiMedUniv, Inst Dermatol, Hefei 230032, Anhui, Peoples R China
AnhuiMedUniv, Dept Dermatol, Hosp 1, Hefei 230032, Anhui, Peoples R China
Key Lab Genome Res Anhui, Hefei 230032, Anhui, Peoples R China
Chinese Natl Human Genome Ctr Shanghai, Shanghai 201203, Peoples R China

Record 22of 46

Alterations in circadian rhythms of melatonin and cortisol in patients with bronchial asthma
Fei GH, Liu RY, Zhang ZH, Zhou JN
ACTA PHARMACOLOGICA SINICA

25 (5): 651-656 MAY 2004

Document type: Article   
Abstract:
AIM: To investigate the possible relationships between alterations in circadian rhythm of melatonin, cortisol and bronchial asthma. METHODS: Salivary melatonin and cortisol were measured simultaneously by radioimmunoassay in 10 mild intermittent or persistent patients, 11 moderate-to-severe persistent asthma patients, and 15 control subjects. Twelve salivary samples were collected in a series during a 24-h period in each subject. RESULTS: The results showed overall lower levels of salivary melatonin in asthma patients compared with control subject (P<0.01). The amplitude, peak-level, and baseline of salivary melatonin were significantly lower in mild intermittent or persistent (P<0.01, P<0.05) and moderate-to-severe persistent asthma patients (P<0.01) compared with control group. The 24-h mean level of salivary cortisol was greatly lower and the acrophase was markedly delayed in patients with mild intermittent or persistent asthma (P<0.01) and moderate-to-severe persistent asthma (P<0.05, P<0.01) compared with control subject. CONCLUSION: Disordered circadian rhythms of salivary melatonin and cortisol were found in asthma patients, which may be involved in the pathogenesis of bronchial asthma.

Author Keywords:
melatonin, asthma, circadian rhythm, glucocorticoids, human, saliva

KeyWords Plus:
NOCTURNAL ASTHMA, SALIVARY MELATONIN, SERUM, INFLAMMATION, HUMANS, PLASMA

Addresses:
Fei GH, AnhuiMedUniv, Affiliated Hosp 1, Dept Pulm, Hefei 230022, Peoples R China
AnhuiMedUniv, Affiliated Hosp 1, Dept Pulm, Hefei 230022, Peoples R China
Univ Sci & Technol China, Sch Life Sci, Anhua 230026, Peoples R China

Record 21of 46

Identification of a locus for punctate palmoplantar keratodermas at chromosome 8q24.13-8q24.21
Zhang XJ, Li M, Gao TW, He PP, Wei SC, Liu JB, Li CR, Cui Y, Yang S, Yuan WT, Li CY, Liu YF, Xu SJ, Huang W
JOURNAL OF INVESTIGATIVE DERMATOLOGY

122 (5): 1121-1125 MAY 2004

Document type: Article   
Abstract:
Punctate palmoplantar keratodermas (PPK) is a rare autosomal dominant cutaneous disorder characterized by numerous hyperkeratotic papules that are irregularly distributed on the palms and soles. The genetic basis for this disease is unknown. We performed a genome-wide search in two Chinese families with punctate PPK to map the chromosome location of the responsible gene. We identified a locus at chromosome 8q24.13-8q24.21 with a cumulative maximum two-point LOD score of 5.41 at markers D8S1793 and D8S1774 (at recombination fraction theta=0.00). Haplotype analysis indicated that the disease gene is located within 9.20 cM region between markers D8S1804 and D8S1720. It is the first locus identified for the punctate PPK. This study provides a map location for isolation of a disease gene-causing punctate PPK.

Author Keywords:
gene mapping, genome-wide scan, linkage analysis, punctate palmoplantar keratodermas

KeyWords Plus:
BUSCHKE-FISCHER-BRAUER, PACHYONYCHIA-CONGENITA, GENE, MUTATIONS, PLANTARIS, LINKAGE, LOCALIZATION, KERATIN-16, MALIGNANCY, FAMILIES

Addresses:
Zhang XJ, AnhuiMedUniv, Inst Dermatol, 69 Meishan Rd, Hefei 230032, Anhui, Peoples R China
AnhuiMedUniv, Inst Dermatol, Hefei 230032, Anhui, Peoples R China
AnhuiMedUniv, Hosp 1, Dept Dermatol, Hefei, Peoples R China
Key Lab Genome Res Anhui, Hefei, Peoples R China
Chinese Natl Human Genome Ctr Shanghai, Shanghai, Peoples R China
Fourth Mil Med Univ, Xijing Hosp, Ctr Dermatol Chinese PLA, Xian, Peoples R China

Record 20of46

Paternal smoking and pregnancy loss: A prospective study using a biomarker of pregnancy
Venners SA, Wang XB, Chen CZ, Wang LH, Chen DF, Guang WW, Huang AQ, Ryan L, O'Connor J, Lasley B, Overstreet J, Wilcox A, Xu XP
AMERICAN JOURNAL OF EPIDEMIOLOGY

159 (10): 993-1001 MAY 15 2004

Document type: Article   
Abstract:
Results of studies on paternal smoking and spontaneous abortions have been inconsistent. The authors examined the effect of paternal smoking on the risk of pregnancy loss in a prospective cohort of 526 newly married, nonsmoking, female textile workers in China between 1996 and 1998. Upon stopping contraception, subjects provided daily urine specimens and records of vaginal bleeding for up to 1 year or until clinical pregnancy. Daily urinary human chorionic gonadotropin was assayed to detect conception and early pregnancy losses, and pregnancies were followed to detect clinical spontaneous abortions. Subjects were grouped by the number of cigarettes that husbands reported smoking daily: nonsmokers (group 1, n = 216), fewer than 20 cigarettes (group 2, n = 239), and 20 or more cigarettes (group 3, n = 71). Compared with that for group 1, the adjusted odds ratio of early pregnancy loss of any conception for group 2 was 1.04 (95% confidence interval (CI): 0.67, 1.63) and for group 3 was 1.81 (95% CI: 1.00, 3.29). The adjusted hazard ratio of conception for group 2 was 0.90 (95% CI: 0.70, 1.18) and for group 3 was 0.96 (95% CI: 0.66, 1.39), while the adjusted hazard ratio of clinical pregnancy for group 2 was 0.93 (95% CI: 0.72, 1.20) and for group 3 was 0.78 (95% CI: 0.55, 1.12). The authors conclude that heavy paternal smoking increased the risk of early pregnancy loss through maternal and/or paternal exposure.

Author Keywords:
abortion, spontaneous, biological markers, chorionic gonadotropin, embryo loss, maternal exposure, prospective studies, smoking, tobacco smoke pollution

KeyWords Plus:
TOBACCO-SMOKE, SPONTANEOUS-ABORTION, CIGARETTE-SMOKING, BIRTH-WEIGHT, EXPOSURE, RISK, REPRODUCTION, ESTROGENS, MODELS

Addresses:
Xu XP, Harvard Univ, Sch Publ Hlth, Dept Environm Hlth, 655 Huntington Ave,FXB-1, Boston, MA 02115 USA
Harvard Univ, Sch Publ Hlth, Dept Environm Hlth, Boston, MA 02115 USA
Boston Univ, Sch Med, Dept Pediat, Boston, MA 02118 USA
Boston Med Ctr, Boston, MA USA
Beijing Med Univ, Ctr Ecogenet & Reprod Hlth, Beijing 100083, Peoples R China
AnhuiMedUniv, Inst Biomed, Anhui, Peoples R China
Harvard Univ, Sch Publ Hlth, Dept Biostat, Boston, MA 02115 USA
Columbia Univ, Coll Phys & Surg, Dept Pathol, New York, NY USA
Columbia Univ, Coll Phys & Surg, Irving Ctr Clin Res, New York, NY USA
Univ Calif Davis, Sch Med, Dept Obstet & Gynecol, Davis, CA 95616 USA
Univ Calif Davis, Inst Toxicol & Environm Hlth, Davis, CA 95616 USA
NIEHS, Epidemiol Branch, NIH, Durham, NC USA

Record 19of 46

Refined localization of dyschromatosis symmetrica hereditaria gene to a 9 center dot 4-cM region at 1q21-22 and a literature review of 136 cases reported in China
He PP, He CD, Cui Y, Yang S, Xu HH, Li M, Yuan WT, Gao M, Liang YH, Li CR, Xu SJ, Chen JJ, Chen HD, Huang W, Zhang XJ
BRITISH JOURNAL OF DERMATOLOGY

150 (4): 633-639 APR 2004

Document type: Article   
Abstract:
Background Dyschromatosis symmetrica hereditaria (DSH) is an autosomal dominant pigmentary genodermatosis characterized by hyperpigmented and hypopigmented macules on the extremities, which has recently been mapped to an 11.6-cM interval on chromosome 1q11-21. So far, most cases of DSH have been reported in Japan and dermatologists around the world might think this disorder mainly occurs in Japan. In fact, there are 17 DSH families including 13 6 cases reported in China since 1980, but most of them are described in Chinese.

Objectives To refine the previously mapped region that facilitates the identification of the DSH gene and to delineate the clinical and genetic features of Chinese DSH cases by a literature review of 136 cases reported in China.

Methods We performed genotyping and linkage analysis using polymorphic microsatellite markers at 1q11-22 in two Chinese DSH families, and reviewed all of the DSH cases reported in China since 1980.

Results A cumulative maximum two-point lod score of 3.68 was produced with marker DIS506 at a recombination frequency of theta = 0.00 in these two families. Haplotype analysis refined the DSH locus to a 9.4-cM interval flanked by D1S2343 and D1S2635. The genetic and clinical features of Chinese cases with DSH were summarized. In some Chinese cases, hyperpigmented and hypopigmented macules were scattered on the neck and chest, but among Japanese patients there were no similar skin lesions to be reported on these sites.

Conclusions This study confirms linkage of DSH to a previously mapped region and refines the DSH gene to a 9.4-cM interval at 1q21-22. Likewise, the literature review indicates that DSH is not an uncommon disorder in China and the differences in the distribution of skin lesions could be related to race and environment.

Author Keywords:
dyschromatosis symmetrica hereditaria, genotyping, haplotype analysis, hyperpigmented and hypopigmented macules, linkage analysis, literature review

KeyWords Plus:
EPIDERMAL DIFFERENTIATION COMPLEX, HUMAN-CHROMOSOME 1Q21, RETICULATE ACROPIGMENTATION, IDENTIFICATION, SCORES, DOHI

Addresses:
Zhang XJ, AnhuiMedUniv, Inst Dermatol, Dept Dermatol, 69 Meishan Rd, Hefei 230032, Peoples R China
AnhuiMedUniv, Inst Dermatol, Dept Dermatol, Hefei 230032, Peoples R China
AnhuiMedUniv, Hosp 1, Key Lab Genome Res, Hefei 230032, Peoples R China
Chinese Natl Human Genome Ctr Shanghai, Shanghai 201203, Peoples R China
China Med Univ, Hosp 1, Dept Dermatol, Shenyang 110001, Liaoning, Peoples R China

Record 18of 46

Effects of astrogalosides on MMP13 and TIMP1 expression in hepatic fibrosis
Wu Q, Yang Y, Chen MZ
SHOCK

21: 62 Suppl. 1 2004

Document type: Meeting Abstract   
Addresses:
AnhuiMedUniv, Dept Pathol, Hefei 230032, Peoples R China

Record 17of 46

Astrogalosides inhibit proliferation of hepatic stellate cells through inducing apoptosis and blocking cell cycle
Wu Q, Yang Y, Chen MZ
SHOCK

21: 113 Suppl. 1 2004

Document type: Meeting Abstract   
Addresses:
AnhuiMedUniv, Dept Pathol, Hefei 230032, Anhui, Peoples R China

Record 16of46

Polymorphsim of monocyte chemattractaxt protein-1 is associated with arthritis of SLE in Han population in China
Ye D, Hu Y, Li X, Yang S, Hao J, Huang F, Zhang X
SHOCK

21: 202 Suppl. 1 2004

Document type: Meeting Abstract   
Addresses:
AnhuiMedUniv, Dept Epidemiol & Biostat, Hefei 230032, Anhui, Peoples R China
Anhui Province Hosp, Dept Rheumatism, Anhui, Peoples R China
AnhuiMedUniv, Inst Dermatol, Anhui, Peoples R China

Record 15of 46

An accidental explosion burn caused by household insecticide
Chen XL
BURNS

30 (3): 289-290 MAY 2004

Document type: Letter   
KeyWords Plus:
AEROSOL

Addresses:
Chen XL, AnhuiMedUniv, Affiliated Hosp 1, Dept Burns, Hefei 230022, Anhui, Peoples R China
AnhuiMedUniv, Affiliated Hosp 1, Dept Burns, Hefei 230022, Anhui, Peoples R China

Record 14of 46

Prevalence of sexual dysfunction in Chinese men with chronic prostatitis
Liang CZ, Zhang XJ, Hao ZY, Shi HQ, Wang KX
BJU INTERNATIONAL

93 (4): 568-570 MAR 2004

Document type: Article   
Abstract:
OBJECTIVE

To investigate the prevalence and risk factors of sexual dysfunction in Chinese men with chronic prostatitis.

PATIENTS AND METHODS

A questionnaire survey was conducted among 2000 men diagnosed as having chronic prostatitis using the National Institutes of Health Chronic Prostatitis Index and analysis of expressed prostatic secretions. The survey was designed to elicit information about age, height, weight, occupation and history of disease and treatment. The erectile capacity of the men was assessed using the five-question version of the International Index of Erectile Function.

RESULTS

Of the 2000 men with chronic prostatitis selected, 1786 completed the survey; the overall prevalence of sexual dysfunction in these patients was 49%. The prevalence of premature ejaculation and erectile dysfunction accounted for 26% and 15%, respectively; 7.7% had both premature ejaculation and erectile dysfunction. There was a negative correlation between prevalence and age, and with the duration of chronic prostatitis (both P < 0.001).

CONCLUSIONS

The prevalence rate of sexual dysfunction in Chinese men with chronic prostatitis is high and related to age.

Author Keywords:
chronic prostatitis, epidemiology, sexual dysfunction

KeyWords Plus:
SYMPTOMS

Addresses:
Liang CZ, AnhuiMedUniv, Affiliated Hosp 1, Dept Urol, Hefei 230022, Peoples R China
AnhuiMedUniv, Affiliated Hosp 1, Dept Urol, Hefei 230022, Peoples R China
AnhuiMedUniv, Inst Dermatol, Hefei 230022, Peoples R China

Record 13of 46

The association between paired basic amino acid cleaving enzyme 4 gene haplotype and diastolic blood pressure
Li JP, Wang XB, Chen CZ, Xu X, Hong XM, Xu XP, Gao W, Huo Y
CHINESE MEDICAL JOURNAL

117 (3): 382-388 MAR 2004

Document type: Article   
Abstract:
Background In a previously identified locus linked to hypertension on chromosome 15q, we identified three blood pressure candidate genes: insulin-like growth factor 1 receptor gene ( IGF1R), myocyte specific enhancer factor 2A gene (MEF2A), and paired basic amino acid cleaving enzyme 4 gene ( PACE4). In this study, we tested their associations with hypertension using haplotype analysis.

Methods A total of 288 unrelated individuals, including 163 high diastolic blood pressure (DBP) subjects and 125 normal IDBP subjects were enrolled in this case-control study. Twenty single nucleotide polymorphisms (SNPs) in the three genes were genotyped using polymerase chain reaction followed by restriction enzyme digestion. Haplotype analysis was accomplished in the following stages: (1) pair-wise linkage disequilibrium test among SNPs on the same gene was performed to explore blocks in which recombination is very unlikely to happen; (2) Estimation-Maximization algorithm was applied to estimate haplotype frequencies in each block; (3) the chi-square test was used to examine the specific haplotype difference, and a permutation test was used to examine the overall haplotype profile difference between cases and controls in each block.

Results An estimated haplotype "CCCCG" frequency in the haplotype block on the PACE4 gene was significantly higher in high DBP cases than in controls ( P < 0.01). The overall estimated haplotype profile in this block was also significantly different between the cases and the controls ( P < 0.001). This association indicates.

Conclusions This study for the first time demonstrated that PACE4 gene may play an important role in the regulation of DBP. This association indicates that variations influencing DBP resides in or near this genomic region.

Author Keywords:
hypertension, paired basic amino acid cleaving enzyme 4, haplotypes

KeyWords Plus:
PROHORMONE CONVERTASES, LINKAGE DISEQUILIBRIUM, IGF-I, FAMILY, POPULATION, ENDOPROTEASE, EXPRESSION, DISEASE, PACE4, IDENTIFICATION

Addresses:
Huo Y, Peking Univ, Hosp 1, Dept Cardiol, Beijing 100034, Peoples R China
Peking Univ, Hosp 1, Dept Cardiol, Beijing 100034, Peoples R China
Boston Univ, Med Ctr, Dept Pediat, Boston, MA 02115 USA
Harvard Univ, Sch Publ Hlth, Program Populat Genet, Boston, MA 02115 USA
AnhuiMedUniv, Inst Biomed, Hefei 230000, Peoples R China

Record 12of 46

Climate variability and transmission of Japanese encephalitis in Eastern China
Bi P, Tong SL, Donald K, Parton KA, Ni JF
VECTOR-BORNE AND ZOONOTIC DISEASES

3 (3): 111-115 FAL 2003

Document type: Article   
Abstract:
A time-series analysis was conducted to study the impact of climate variability on the transmission of Japanese encephalitis in eastern China. Correlation and regression analyses were used to examine the relationship between monthly climatic variables and monthly incidence of Japanese encephalitis in Jieshou County, China over the period 1980-96. Spearman's correlation analysis showed that maximum and minimum temperatures and rainfall were all associated with the transmission of Japanese encephalitis in the county. Regression analysis suggested that monthly mean minimum temperature and monthly precipitation had a significant relationship with the. transmission of Japanese encephalitis, with a 1-month lag effect. The results indicated that these climatic variables might be treated as possible predictors for regions with similar geographic, climatic, and socio-economic conditions to Jieshou County.

Author Keywords:
China, climate, Japanese encephalitis, quantitative analysis

KeyWords Plus:
HEMORRHAGIC-FEVER, RENAL SYNDROME, VARIABLES

Addresses:
Bi P, Univ Adelaide, Dept Publ Hlth, Adelaide, SA 5005, Australia
Univ Adelaide, Dept Publ Hlth, Adelaide, SA 5005, Australia
Queensland Univ Technol, Sch Publ Hlth, Brisbane, Qld, Australia
Univ Sydney, Fac Rural Management, Orange, NSW, Australia
AnhuiMedUniv, Dept Epidemiol, Hefei, Peoples R China

Record 11of 46

Association between the interleukin-1 beta C(-511)T polymorphism and blood pressure in a Chinese hypertensive population
Guo HA, Niu TH, Peng SJ, Ling DJ, Liu J, Zhang XJ, Xu XP
IMMUNOLOGY LETTERS

91 (2-3): 159-162 FEB 15 2004

Document type: Article   
Abstract:
The interleukin-1beta (IL-1beta) is an important pro-inflammatory cytokine in a broad spectrum of physiological processes. Previous investigations have observed that levels of the IL-1beta were higher inessential hypertensive patients and the IL-1beta gene polymorphism has been shown to be related to IL-1 production. We hypothesized that genetically determined differences in activity or responsiveness of cytokine(s) might contribute to hypertension. In this report, we utilized a family-based design to test the association between the IL-1beta C(-511)T polymorphism and blood pressure levels in hypertensive patients chosen from rural communities in Anhui, China. In men, carriers of the IL-1beta (-511)*C allele were found to have lower systolic (P = 0.049) blood pressure levels compared with T homozygotes, which conforms to an additive effect model. By contrast, no significant association between the IL-1beta gene and blood pressure levels was revealed in women. Our results suggested a significant role of the IL-1beta C(-511)T polymorphism in the control of blood pressure in Chinese hypertensives. (C) 2003 Elsevier B.V. All rights reserved.

Author Keywords:
interleukin-1 beta, polymorphism, blood pressure, hypertension, family based association test

KeyWords Plus:
RECEPTOR ANTAGONIST GENE, TUMOR-NECROSIS-FACTOR, PERIPHERAL-BLOOD, DISEASE, EXPRESSION, GENOTYPE, CELLS, CYTOKINES, IL-1RA

Addresses:
Xu XP, Univ Sci & Technol China, Sch Life Sci, Hefei 230026, Peoples R China
Univ Sci & Technol China, Sch Life Sci, Hefei 230026, Peoples R China
AnhuiMedUniv, Dept Dermatol, Hefei, Peoples R China
Harvard Univ, Sch Publ Hlth, Program Populat Genet, Boston, MA 02115 USA
Anhui Inst Biomed, Hefei, Peoples R China

Record 10of 46

Diurnal rhythms of free estradiol and cortisol during the normal menstrual cycle in women with major depression
Bao AM, Ji YF, Van Someren EJW, Hofman MA, Liu RY, Zhou JN
HORMONES AND BEHAVIOR

45 (2): 93-102 FEB 2004

Document type: Article   
Abstract:
To investigate whether depression is accompanied by changes in diurnal rhythms of free estradiol and cortisol in different phases of the menstrual cycle, we measured these two hormone levels in saliva samples collected every 2 It for 24 It from 15 healthy normally cycling women and 12 age-matched normally cycling women suffering from major depression taking antidepressants. The assessments were repeated four times over one menstrual cycle: during menstruation and in the late follicular/peri-ovulating, early to mid-luteal and late luteal phases, respectively. Quantification with a nonlinear periodic regression model revealed distinct diurnal rhythms in free estradiol and free cortisol in all subjects. For the diurnal cortisol rhythm, significant differences were found in the peak-width and ultradian amplitude among different menstrual phases, both in controls and depressed patients, while no significant differences were found between the two groups. The diurnal estradiol rhythm, on the other hand, was quite consistent among different menstrual phases within both groups, while the depressed patients had overall larger amplitudes than controls, which is negatively correlated with disease duration. Significant positive correlations between the two hormone rhythms were found for 24-h mean level (mesor, peak, and trough in late luteal phase, and for ultradian harmonics in early to mid-luteal phase in controls, but only for ultradian harmonics in late follicular/peri-ovulating phase and for acrophase in the menstruation phase in depressed patients. A sub-analysis was also performed in patients who received Fluoxetine (n = 7). The findings implicate a close correlation between the hypothalamic -pituitary - adrenal axis and the hypothalamic - pituitary - gonadal axis, both of which may be involved in depression. (C) 2004 Elsevier Inc. All rights reserved.

Author Keywords:
major depression, diurnal rhythm, ultradian rhythm, free estradiol, free cortisol, menstrual cycle

KeyWords Plus:
CORTICOTROPIN-RELEASING-FACTOR, POSTTRAUMATIC-STRESS-DISORDER, PITUITARY-GONADAL AXIS, SUPRACHIASMATIC NUCLEUS, PARAVENTRICULAR NUCLEUS, GONADOTROPIN-SECRETION, MOOD DISORDERS, HORMONE LEVELS, EXCRETION, NEURONS

Addresses:
Zhou JN, Univ Sci & Technol China, Sch Life Sci, Dept Neurobiol, Hefei 230032, Anhui, Peoples R China
Univ Sci & Technol China, Sch Life Sci, Dept Neurobiol, Hefei 230032, Anhui, Peoples R China
AnhuiMedUniv, Affiliated Hosp 1, Anhui Geriatr Inst, Hefei 230032, Anhui, Peoples R China
Psychiat Ctr Anhui Prov, Hefei 230032, Anhui, Peoples R China
Psychiat Hosp Hefei City, Hefei 230032, Anhui, Peoples R China
Netherlands Inst Brain Res, NL-1105 AZ Amsterdam, Netherlands
VU Univ Med Ctr, Inst Clin & Expt Neurosci, NL-1081 HV Amsterdam, Netherlands

Record 9of 46

HLA-DQA1 and DQB1 alleles are associated with genetic susceptibility to psoriasis vulgaris in Chinese Han
Zhang XJ, Wei SC, Yang S, Wang ZX, Zhang AP, He PP, Wang HY
INTERNATIONAL JOURNAL OF DERMATOLOGY

43 (3): 181-187 MAR 2004

Document type: Article   
Abstract:
Background Psoriasis vulgaris is a chronic skin disorder characterized by infiltration of inflammatory elements, keratinocyte hyperproliferation and altered differentiation. Although the pathogenesis of psoriasis is not fully understood, there is solid evidence of a susceptibility locus in the human leukocyte antigen (HLA) region.

Objectives To investigate whether HLA-DQA1 and DQB1 alleles are associated with genetic susceptibility to psoriasis vulgaris in Chinese Han.

Patients and methods The polymerase chain reaction-sequence-specific primer (PCR-SSP) method was used to analyse the distribution of HLA-DQA1 and DQB1 alleles in 189 patients with psoriasis and 273 healthy controls.

Results The HLA-DQA1*0104 (OR = 2.33, P = 0.0001154, P-c = 2.0 x 10(-3)), DQA1*0201 (OR = 3.36, P < 1.0 x 10(-7), P-c < 1.0 x 10(-6)), DQB1*0201 (OR = 1.64, P = 0.0192, P-c > 0.05) and DQB1*0303 (OR = 1.55, P = 0.0377, P-c > 0.05) alleles were more prevalent in patients with psoriasis vulgaris than in controls, and HLA-DQA1*0501 (OR = 0.30, P = 0.0000039, P-c < 4.0 x 10(-5)) alleles were less prevalent. The HLA-DQA1*0104 (OR = 2.42, P = 0.0001159, P-c < 2.0 x 10(-3)), DQA1*0201 (OR = 3.74, P < 1.0 x 10(-7), P-c < 1.0 x 10(-6)) and DQA1*0501 (OR = 0.30, P = 0.0000374, P-c < 4.0 x 10(-4)) alleles were only associated with type I psoriasis. HLA-DQA1*0104 and DQA1*0201 were more prevalent in patients with or without a family history of psoriasis. However, the DQA1*0501 allele was only more prevalent in patients without a family history of psoriasis.

Conclusion HLA-DQA1*0104 and DQA1*0201 alleles may be psoriasis susceptibility genes or may be in close linkage with the susceptibility genes. The HLA-DQA1*0501 allele seems to have a protective effect against the development of psoriasis vulgaris in Chinese Han. There may be a difference in genetic background between psoriasis patients with and without a family history of psoriasis.

KeyWords Plus:
GENOME-WIDE SCAN, CHROMOSOME 6P, HLA-ANTIGENS, LOCUS, HAPLOTYPE, 17Q

Addresses:
Zhang XJ, AnhuiMedUniv, Inst Dermatol, Hefei 230022, Peoples R China
AnhuiMedUniv, Inst Dermatol, Hefei 230022, Peoples R China

Record 8of 46

Hypertensive patients from two rural Chinese counties respond differently to benazepril: The Anhui hypertension health care study
Li D, Xing HX, Hao K, Peng SJ, Wu D, Guang WW, Huang AQ, Wang YP, Zhang Y, Yu YX, Li JP, Chen CZ, Wang BY, Zhu GY, Huo Y, Chen DF, Ronnenberg A, Niu TH, Xu XP
ANNALS OF EPIDEMIOLOGY

14 (2): 123-128 FEB 2004

Document type: Article   
Abstract:
PURPOSE: Essential hypertension, as a complex disorder with unknown etiology cause, is a major public health problem worldwide. Patients need constant drug therapy to maintain their blood pressure in a normal range. However, the current facts suggest that the treatment is not optimized in a large number of patients, and as a result they are at risk for compliance resulting in uncontrolled blood pressure. Genetic and environmental factors associated with individual variation in response to anti-hypertensive drug remain largely unknown.

METHODS: In order to illustrate the existence and to attempt to identify the factors modifying drug effect, we conducted a large-scale follow-up study in two Chinese rural counties differing in both genetic background and residential environment. Hypertensive patients were treated with benazepril, a commonly used angiotensin converting enzyme inhibitor, for 15 days, and the end-point effect was evaluated.

RESULTS: We found that there were large and significant differences in drug response between subjects from two counties, even after adjustment for known factors. The responses to benazepril, measured in diastolic blood pressure drop, in male patients from Yuexi was twice as effective as their counterparts from Huoqiu.

CONCLUSIONS: These results suggest that adjustment of treatment regimen is necessary to improve efficacy, and it could be done at the population level to make it more feasible and affordable. (C) 2003 Elsevier Inc. All rights reserved.

Author Keywords:
hypertension, benazepril, response, pharmacoepidemiology

KeyWords Plus:
BLOOD-PRESSURE, ADULTS

Addresses:
Xu XP, Harvard Univ, Sch Publ Hlth, Program Populat Genet, 665 Huntington Ave,FXB-101, Boston, MA 02115 USA
Harvard Univ, Sch Publ Hlth, Program Populat Genet, Boston, MA 02115 USA
Peking Univ, Affiliated Hosp 1, Dept Cardiovasc Dis, Beijing 100871, Peoples R China
Peking Univ, Sch Med, Dept Genet & Cell Biol, Ctr Ecogenet & Reprod Hlth, Beijing, Peoples R China
AnhuiMedUniv, Inst Biomed, Hefei, Peoples R China
Univ Sci & Technol China, Sch Life Sci, Hefei 230026, Peoples R China

Record 7of 46

A gene for freckles maps to chromosome 4q32-q34
Zhang XJ, He PP, Liang YH, Yang S, Yuan WT, Xu SJ, Huang W
JOURNAL OF INVESTIGATIVE DERMATOLOGY

122 (2): 286-290 FEB 2004

Document type: Article   
Abstract:
Freckles are numerous pigmented macules on the face commonly occurring in the Caucasian and Chinese population. As freckling is considered as an independent trait, no gene or locus for it has been identified to date. Here we performed genome-wide scan for linkage analysis in a multigeneration Chinese family with freckles. A maximum LOD score of 4.26 at a recombination fraction of 0 has been obtained with marker D4S1566. Haplotype analysis localized the freckles locus to a 16 Mbp region flanked by D4S2952 and D4S1607. We have thus mapped the gene for freckles to chromosome 4q32-q34. This will aid future identification of the responsible gene, which will be very useful for the understanding of the molecular mechanism of freckles.

Author Keywords:
freckles, gene mapping, genome-wide scan, linkage analysis

KeyWords Plus:
DYSCHROMATOSIS SYMMETRICA HEREDITARIA, TRANSCRIPTION FACTOR, IDENTIFICATION, MELANOSOMES, BIOGENESIS, PROTEINS, PALLIDIN, SCORES, DHAND, LOCUS

Addresses:
Zhang XJ, AnhuiMedUniv, Inst Dermatol, 69 Meishan Rd, Hefei 230032, Anhui, Peoples R China
AnhuiMedUniv, Inst Dermatol, Hefei 230032, Anhui, Peoples R China
AnhuiMedUniv, Hosp 1, Dept Dermatol, Hefei 230032, Anhui, Peoples R China
Chinese Natl Human Genome Ctr Shanghai, Shanghai 201203, Peoples R China
Key Lab Genome Res Anhui, Hefei, Peoples R China

Record 6of 46

Regional alteration of synapsin I in the hippocampal formation of Alzheimer's disease patients
Qin S, Hu XY, Xu H, Zhou JN
ACTA NEUROPATHOLOGICA

107 (3): 209-215 MAR 2004

Document type: Article   
Abstract:
Synapsin I is a synaptic vesicle-associated protein, participating in synapse formation, regulation of the synthesis of other synaptic vesicle proteins and promotion of neurotransmitter release. The aim of present study was to detect whether regional alteration of synapsin I occurred in the hippocampal formation of Alzheimer's disease (AD) patients. Samples of hippocampus from ten female AD patients and ten non-demented female controls, matched for age and postmortem delay, were investigated immunocytochemically. Using immunofluorescence labeling and laser confocal imaging, we examined the distribution of synapsin I in the CA1 and CA3 subfields of the hippocampus and the dentate gyrus (DG) in AD patients and controls. The results showed that synapsin I was significantly decreased in the stratum radiatum of CA1 subfield and the molecular layer of DG in AD patients. No difference between the two groups in the intensity of synapsin I staining was observed in the stratum pyramidale of CA1, where a number of pretangles stained by Alz-50 were observed in AD patients. These results suggest that there is regional loss of synapsin I in the hippocampal formation of AD patients.

Author Keywords:
Alzheimer's disease, hippocampus, synapsin I, laser-scanning confocal microscopy, immunoreactivity

KeyWords Plus:
SYNAPTIC VESICLE, NEUROFIBRILLARY TANGLES, COGNITIVE DECLINE, DEFICIENT MICE, SENILE PLAQUES, GENE FAMILY, IMPAIRMENT, PROTEINS, NEURONS, PHOSPHOPROTEIN

Addresses:
Zhou JN, Univ Sci & Technol China, Sch Life Sci, Dept Neurobiol, POB 4, Hefei 230027, Anhui, Peoples R China
Univ Sci & Technol China, Sch Life Sci, Dept Neurobiol, Hefei 230027, Anhui, Peoples R China
AnhuiMedUniv, Affiliated Hosp 1, Anhui Geriatr Inst, Hefei 230022, Anhui, Peoples R China

Record 5of 46

beta 3 adrenergic receptor polymorphism and obesity-related phenotypes in hypertensive patients
Hao K, Peng SJ, Xing HX, Yu YX, Huang AQ, Hong XM, Wang YP, Chen CZ, Wang BY, Zhang XJ, Liu J, Zhu GY, Huo Y, Chen DF, Zhao XT, Ronnenberg A, Wu D, Niu T, Xu XP
OBESITY RESEARCH

12 (1): 125-130 JAN 2004

Document type: Article   
Abstract:
Objectives: Obesity is a complex trait that is affected by both environmental and genetic risk factors. The 03 adrenergic receptor (ADRB3) is expressed in adipose tissue and plays a role in energy metabolism. A missense mutation on codon 64 of this gene (W64R) is associated with receptor malfunction. Previous studies examining the relation between this polymorphism and obesity produced inconsistent findings. The current study assessed the association between the W64R genotype and obesity-related phenotypes, including body weight, BMI, and serum triglycerides, cholesterol, and glucose.

Research Methods and Procedures: We determined the ADRB3 W64R genotypes and fasting serum lipid and glucose concentrations for 695 hypertensive adults (336 men, 359 women) from a rural county in Anhui Province, China. Multivariate linear regression models were fit to detect associations between the genetic polymorphism and obesity-related phenotypes.

Results: The ADRB3 W64R polymorphism was significantly associated with body weight and BMI in men but not in women. After controlling for potential confounding variables, men who were homozygous for the R64 allele were 11.8 kg heavier (p < 0.001) and had a BMI that was 3.7 kg/m(2) greater (p = 0.001) than men who were homozygous for the W64 allele. Serum concentrations of lipids and glucose were found not associated with the genetic polymorphism.

Discussion: The ADRB3 R64 allele was associated with increased body weight and BMI in men but not in women. The genetic association was not modified by triglyceride, cholesterol, blood glucose, or blood pressure levels of the subjects.

Author Keywords:
beta 3 adrenergic receptor, polymorphism, obesity phenotypes, gender

KeyWords Plus:
BETA(3)-ADRENERGIC RECEPTOR GENE, BETA-3 ADRENERGIC-RECEPTOR, TRP64ARG POLYMORPHISM, INSULIN-RESISTANCE, DIABETES-MELLITUS, ASSOCIATION, MUTATION, WEIGHT, POPULATION, VARIANT

Addresses:
Xu XP, Harvard Univ, Sch Publ Hlth, Program Populat Genet, 665 Huntington Ave,FXB-101, Boston, MA 02115 USA
Harvard Univ, Sch Publ Hlth, Program Populat Genet, Boston, MA 02115 USA
AnhuiMedUniv, Inst Biomed, Hefei, Peoples R China
Univ Sci & Technol China, Sch Life Sci, Hefei 230026, Peoples R China
Peking Univ, Sch Med, Dept Genet & Cell Biol, Ctr Eco Genet & Reprod Hlth, Beijing 100871, Peoples R China
Peking Univ, Affiliated Hosp 1, Dept Cardiovasc Dis, Beijing 100871, Peoples R China

Record4of 46

Epidermal growth factor activates store-operated Ca2+ channels through an inositol 1,4,5-trisphosphate-independent pathway in human glomerular mesangial cells
Li WP, Tsiokas L, Sansom SC, Ma R
JOURNAL OF BIOLOGICAL CHEMISTRY

279 (6): 4570-4577 FEB 6 2004

Document type: Article   
Abstract:
One of the fastest cellular responses following activation of epidermal growth factor receptor is an increase in intracellular Ca2+ concentration. This event is attributed to a transient Ca2+ release from internal stores and Ca2+ entry from extracellular compartment. Store-operated Ca2+ channels are defined the channels activated in response to store depletion. In the present study, we determined whether epidermal growth factor activated store-operated Ca2+ channels and further, whether depletion of internal Ca2+ stores was required for the epidermal growth factor-induced Ca2+ entry in human glomerular mesangial cells. We found that 100 nM epidermal growth factor activated a Ca2+-permeable channel that had identical biophysical and pharmacological properties to channels activated by 1 muM thapsigargin in human glomerular mesangial cells or A431 cells. The epidermal growth factor-induced Ca2+ currents were completely abolished by a selective phospholipase C inhibitor, U73122. However, xestospongin C, a specific inositol 1,4,5-trisphosphate receptor inhibitor, did not affect the membrane currents elicited by epidermal growth factor despite a slight reduction in background currents. Following emptying of internal Ca2+ stores by thapsigargin, epidermal growth factor still potentiated the Ca2+ currents as determined by the whole-cell patch configuration. Furthermore, epidermal growth factor failed to trigger measurable Ca2+ release from endoplasmic reticulum. However, another physiological agent linked to phospholipase C and inositol 1,4,5-trisphosphate cascade, angiotensin II, produced a striking Ca2+ transient. These results indicate that epidermal growth factor activates store-operated Ca2+ channels through an inositol 1,4,5-trisphosphate-independent, but phospholipase C-dependent, pathway in human glomerular mesangial cells.

KeyWords Plus:
CAPACITATIVE CALCIUM-ENTRY, CATION CHANNELS, A431 CELLS, TRISPHOSPHATE RECEPTOR, SIGNAL-TRANSDUCTION, INTRACELLULAR CA2+, ANGIOTENSIN-II, HTRP3 CHANNELS, SMOOTH-MUSCLE, B-LYMPHOCYTES

Addresses:
Tsiokas L, Univ Oklahoma, Hlth Sci Ctr, Dept Cell Biol, 940 Stanton L Young Blvd, Oklahoma City, OK 73190 USA
Univ Oklahoma, Hlth Sci Ctr, Dept Cell Biol, Oklahoma City, OK 73190 USA
AnhuiMedUniv, Dept Pharmacol, Hefei, Peoples R China
Univ Nebraska, Med Ctr, Dept Physiol & Biophys, Omaha, NE 68198 USA

Record3of 46

Activation of ERK1/2 MAP kinase pathway induces tight junction disruption in human corneal epithelial cells
Wang Y, Zhang J, Yi XJ, Yu FSX
EXPERIMENTAL EYE RESEARCH

78 (1): 125-136 JAN 2004

Document type: Article   
Abstract:
Activation of protein kinase C (PKC) by exposure of cultured human corneal epithelial cells to phorbol 12-myristate 13-acetate (PMA) resulted in an increase in paracellular permeability as evidenced by a decrease in transepithelial electrical resistance (TER). A change in the membrane distribution of the tight junction protein ZO-1 was also observed in the PMA-treated cells. In contrast, when the cells were treated with PMA in the presence of PD98059, a specific inhibitor of mitogen-activated protein kinase (MAPK) kinase, all barrier characteristics were preserved, suggesting that PKC induces tight junction disruption through the activation of MAPK. The role of this signaling pathway in the regulation of epithelial permeability was further elucidated by the use of corneal epithelial-derived cell lines expressing constitutively activated (ca) or dominant-negative (dn) mutants of MAPK kinase-1 (MEK1). Transfectants of caMEK1, when compared to parental cells, had higher levels of phosphorylated extracellular regulated protein kinase (ERK), altered distribution of ZO-1 and occludin, and much reduced TER. On the other hand, dnMEK1 transfectants had lower but detectable levels of ERK phosphorylation, more flattened morphology, and, most importantly, significantly higher TER when compared to parental cells. Our study demonstrates that activation of PKC causes the disruption of tight junctions through activation of MAP kinase and that the MAP kinase signaling pathway plays a key role in the regulation of epithelial cell morphology and barrier function in the cornea. (C) 2004 Elsevier Ltd. All rights reserved.

Author Keywords:
corneal epithelium, tight junction, paracellular permeability, protein kinase C, the mitogen-activated protein kinase

KeyWords Plus:
INTESTINAL BARRIER FUNCTION, CANINE KIDNEY-CELLS, PROTEIN-KINASE, ZONULA OCCLUDENS, MAMMALIAN-CELLS, C ACTIVATION, ZO-1, PERMEABILITY, PHOSPHORYLATION, LOCALIZATION

Addresses:
Yu FSX, Med Coll Georgia, Dept Cellular Biol & Anat, Augusta, GA 30912 USA
Med Coll Georgia, Dept Cellular Biol & Anat, Augusta, GA 30912 USA
AnhuiMedUniv, Mol Biol Lab, Hefei 230032, Anhui, Peoples R China

Record 2 of 46

Free radical scavenging activity of extracts prepared from fresh leaves of selected Chinese medicinal plants
Hu FL, Lu RL, Huang B, Ming L
FITOTERAPIA

75 (1): 14-23 JAN 2004

Document type: Article   
Abstract:
The free radical scavenging activity of the 80% methanolic extracts from fresh leaves of 300 Chinese medical woody plants was assessed with the aid of the stable DPPH radical. Among the plants screened, 56 species had strong free radical scavenging capacities, with IC50 values lower than 0.5 mg leaves per milliliter. Analysis of the medical uses of these plants showed that most of them are employed for their effects on hemostasis, as antiinflammatory, antimicrobial or for treatment of dysentery. These uses may be directly linked to the content in tannins and flavonoids and consequently to their free radical scavenging activities. (C) 2003 Elsevier B.V. All rights reserved.

Author Keywords:
Chinese medicinal plants, free radical scavenging activity

KeyWords Plus:
FLAVONOIDS, JAPONICA

Addresses:
Hu FL, Anhui Agr Univ, Hefei 230036, Peoples R China
Anhui Agr Univ, Hefei 230036, Peoples R China
AnhuiMedUniv, Hefei 230036, Peoples R China

Record 1 of 46

Distribution and expression of non-muscle myosin light chain kinase in rabbit livers
Zhu HQ, Wang Y, Hu RL, Ren B, Zhou Q, Jiang ZK, Gui SY
WORLD JOURNAL OF GASTROENTEROLOGY

9 (12): 2715-2719 DEC 2003

Document type: Article   
Abstract:
AIM: To study the distribution and expression of non-muscle myosin light chain kinase (nmMLCK) in rabbit livers.

METHODS: Human nmMLCK N-terminal cDNA was amplified by polymerase chain reaction (PCR) and was inserted into pBKcmv to construct expression vectors. The recombinant plasmid was transformed into XL1-blue. Expression protein was induced by IPTG and then purified by SDS-PAGE and electroelution, which was used to prepare the polycolonal antibody to detect the distribution and expression of nmMLCK in rabbit livers with immunofluorescene techniques.

RESULTS: The polyclonal antibody was prepared, by which nmMLCK expression was detected and distributed mainly in peripheral hepatocytes.

CONCLUSION: nmMLCK can express in hepatocytes peripherally, and may play certain roles in the regulation of hepatic functions.

KeyWords Plus:
SECRETORY CASCADE, PROTEIN-KINASE, PHOSPHORYLATION, LOCALIZATION, CELL, ORGANIZATION, CONTRACTION, ACTIVATION, ENTRY, RAT

Addresses:
Wang Y, AnhuiMedUniv, Mol Biol Lab, Hefei 230032, Anhui, Peoples R China
AnhuiMedUniv, Mol Biol Lab, Hefei 230032, Anhui, Peoples R China
AnhuiMedUniv, Dept Biochem, Hefei 230032, Anhui, Peoples R China
Anhui Prov Key Lab Genom Res, Hefei 230032, Anhui, Peoples R China
BIDMC, Dept Pathol, Boston, MA 02215 USA
Harvard Univ, Sch Med, Boston, MA 02215 USA
AnhuiMedUniv, Affiliated Hosp 1, Dept Resp Dis, Hefei 230032, Anhui, Peoples R China
PLA, Hosp 105, Hefei 230031, Anhui, Peoples R China